Coding

Part:BBa_K2926050:Design

Designed by: Johanna Opgenoorth   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


Fusion protein of Flo11 from yeast and mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To enable proper protein folding of both subdomains we inserted a short glycine-serine-linker in between them.


Source

The n-terminal domain of Flo11 was obtained via gene synthesis while mCherry was isolated from the iGEM part BBa_J06504.

References